Description
>Our Caspase-10 Assay Kit, Colorimetric provides a simple and convenient method for assaying the activity of caspases 10, which is based on the hydrolysis of the labeled substrate Ac-AEVD-pNA (N-Acetyl-Ala-Glu-Val-Asp-p-nitroanilide) by Caspase-10, releasing the pNA (p-nitroaniline) moiety from the substrate. The released pNA has the max absorbance at 405 nm (εmM = 10.5) and its concentration is calculated by measuring the absorbance values at 405 nm or from a standard calibration curve prepared using the pNA Dye Standard, using a microplate reader or spectrophotometer